Munotec proteins are non-human, non-mammalian fusion proteins that bind to the Fab of antibodies (e.g., IgG, IgA and IgM) of various species and block the antigen binding site, thereby mimicking the antigen.
Munotec proteins can:
serve as a surrogate antigen
neutralize antibody binding to antigen
capture and orient antibodies on solid matrices
differentiate antigen-engaged Fab from non-engaged Fab
confirm antibody binding and target specificity (see below)
detect antibodies in solution or in a solid matrix (e.g., bead, blot, wells, resin)
Enzyme (e.g., HRP)-Munotec or tag-Munotec proteins are available and offer limitless applications. Customized Munotec constructs are available through a non-exclusive licensing agreement with ITBS LLC.
Data sample A: Munotec as an immunologic characterization tool
Soluble Munotec is mixed with a monoclonal antibody (at 50X and 25X eq. molar concentration) to promote binding thereby blocking binding of the antibody to its target antigen (immobilized on wells) and therefore a) demonstrate that binding is Fab dependent and b) confirming the target specificity of the antibody.
Data sample B: Munotec serves as a surrogate antigen
Mono-biotinylated Munotec (immobilized on streptavidin wells) serves as a surrogate antigen and is used to confirm antibody presence in solution. A secondary antibody recognizing the antibody heavy chain, labeled with HRP (horseradish peroxidase) was used to detect the Munotec-bound antibody.
Munotec proteins are For Research Use Only. Not for use in diagnostic procedures.